C型钠肽预处理对牛卵母细胞体外成熟的影响

doi:10.11843/j.issn.0366-6964.2014.09.007

Abstract

Abstract:

To study the in vitro maturation (IVM) of bovine oocyte pretreated with C-type natriuretic peptide (CNP).Compared with the conventional 24 h IVM culture，the effect of bovine oocytes pre-treated with CNP for 6 h following 28 h IVM culture (CNP pre-treated group) were studied.The results showed that the maturation of bovine oocyte in CNP pre-treated group were significantly improved，and the blastocyst rate and the cell number of blastocyst were significantly higher than those of the control group（(51.55%±2.50%)，（156.2±11.0）vs.（28.13%±3.80%）,(116.3±19.0)，P<0.05) after in vitro fertilization (IVF).The distribution of mitochondria ranged from peripheral distribution in unmatured oocyte to homogenous distribution throughout the cytoplasm in matured oocytes，while semi-peripheral distribution observed in the oocytes pretreated with CNP for 6 h，but then became homogenous distribution followed 28 h maturation culture.The mtDNA copy number of matured oocyte in CNP pre-treated group were (510 078.4±28 906.0)，which was significantly higher than that of control group((416 558.5±51 743.0)，P<0.05).The results indicated that the quality of bovine oocytes in vitro maturation pre-treated for 6 h with CNP could be improved effectively.

CLC Number:

S823.3

ZHANG Chun-qiang，OUYANG Xiao-qing，ZHANG Tong，LI Rui-lan，FAN Xiao-mei，ZHANG Jia-xin. Effects of C-type Natriuretic Peptide Pretreatment on in vitro Maturation of Bovine Oocyte[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1424-1431.

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Effects of C-type Natriuretic Peptide Pretreatment on in vitro Maturation of Bovine Oocyte

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